Bethyl Laboratories
Rabbit anti-CAP-H2 Antibody Affinity Purified
Primary
Catalog #A302-276A
TargetCAP-H2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CAP-H2 (chromosome-associated protein H2) is 1 of 3 non-SMC subunits that define the condensin II complex. Condensin II along with the condensin I plays a critical role in mitotic chromosome assembly and segregation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 555 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCAP-H2
Antigen Species
Human
Gene ID29781
Gene Symbol
NCAPH2
Gene Name
non-SMC condensin II complex subunit H2
Uniprot IDQ6IBW4
Protein Name
Condensin-2 complex subunit H2
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CAP-H2 immobilized on solid support.
The epitope recognized by A302-276A maps to a region between residue 555 and 605 of human chromosome-associated protein H2 using the numbering given in entry NP_689512.2 (GeneID 29781).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-276A · Lot 2 (current lot)Open
Product DatasheetPDF · A302-276A · Lot 2 (current lot)Open
Safety Data SheetPDF · A302-276AOpen
Citations / Publications
Services
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