/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CARMIL Antibody
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Primary Antibodies  

Rabbit anti-CARMIL Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CARMIL

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1321 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Country of Origin

USA

Additional Product Information

CARMIL binds CAPZA2 with high affinity and significantly decreases CAPZA2 affinity for actin barbed ends. It increases the rate of elongation from seeds in the presence of CAPZA2, however, seems unable to nucleate filaments. CARMIL Rapidly uncaps barbed ends capped by CAPZA2 and enhances barbed-end actin polymerization (By similarity).CARMIL may control actin dynamics in lamellipodi and be required for cell migration [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q5VZK9].

55604

CARMIL1

capping protein regulator and myosin 1 linker 1

Q5VZK9

F-actin-uncapping protein LRRC16A

Alternate Names

capping protein regulator and myosin 1 linker protein 1; capping protein, Arp2/3 and myosin-I linker homolog 1; capping protein, Arp2/3 and myosin-I linker protein 1; capping protein, Arp2/3, and Myosin-I Linker homolog 1; CARMIL; CARMIL homolog; CARMIL1a; dJ501N12.1; dJ501N12.5; F-actin-uncapping protein LRRC16A; leucine rich repeat containing 16; leucine rich repeat containing 16A; leucine-rich repeat-containing protein 16A; LRRC16; LRRC16A; testicular tissue protein Li 107

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.