Bethyl Laboratories
Rabbit anti-CASC3 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00690
TargetCASC3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
Cancer susceptibility candidate gene 3 protein (CASC3) is a core component of the exon junction complex (EJC), a protein complex that is deposited on spliced mRNAs at exon-exon junctions and functions in nonsense-mediated mRNA decay (NMD). The encoded protein binds RNA and interacts with two other EJC core components. CASC3 is predominantly located in the cytoplasm, but shuttles into the nucleus where it localizes to nuclear speckles [taken from NCBI Entrez Gene (GeneID: 22794)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 653 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCASC3
Antigen Species
Human
Gene ID22794
Gene Symbol
CASC3
Gene Name
CASC3, exon junction complex subunit
Uniprot IDO15234
Protein Name
Protein CASC3
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CASC3 immobilized on solid support.
The epitope recognized by IHC-00690 maps to a region between residue 653 and 703 of human cancer susceptibility candidate 3 using the numbering given in entry NP_031385.2 (GeneID 22794).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00690Open
Product DatasheetPDF · IHC-00690 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00690 · Lot 1 (current lot)Open
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