Bethyl Laboratories
Rabbit anti-Cbl-b Antibody Affinity Purified
Primary
Catalog #A302-902A
TargetCbl-b
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
Cbl-b is an E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Cbl-b negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q13191].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCbl-b
Antigen Species
Human
Gene ID868
Gene Symbol
CBLB
Gene Name
Cbl proto-oncogene B
Uniprot IDQ13191
Protein Name
E3 ubiquitin-protein ligase CBL-B
Alternate Names
Cas-Br-M (murine) ecotropic retroviral transforming sequence b • casitas B-lineage lymphoma proto-oncogene b • Cbl proto-oncogene B, E3 ubiquitin protein ligase • Cbl proto-oncogene, E3 ubiquitin protein ligase B • Cbl-b • E3 ubiquitin-protein ligase CBL-B • Nbla00127 • RING finger protein 56 • RING-type E3 ubiquitin transferase CBL-B • RNF56 • SH3-binding protein CBL-B • signal transduction protein CBL-B
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Cbl-b immobilized on solid support.
The epitope recognized by A302-902A maps to a region between residue 450 and 500 of human Cas-Br-M (murine) Ecotropic Retroviral Transforming Sequence b using the numbering given in entry NP_733762.2 (GeneID 868).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-902A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-902A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-902AOpen
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