Bethyl Laboratories
Rabbit anti-CCDC16 Antibody Affinity Purified
Primary
Catalog #A301-418A
TargetCCDC16
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CCDC16 (coiled-coil domain containing 16) is a U1-like C2H2-type zinc finger protein whose function has not been characterized. Coiled-coil proteins exhibit tertiary structures made up of a number of highly organized alpha helices wound around each other to mediate oligomerization.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCCDC16
Antigen Species
Human
Gene ID91603
Gene Symbol
ZNF830
Gene Name
zinc finger protein 830
Uniprot IDQ96NB3
Protein Name
Zinc finger protein 830
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CCDC16 immobilized on solid support.
The epitope recognized by A301-418A maps to a region between residue 150 and 200 of human coiled-coil domain containing 16 using the numbering given in entry NP_443089.3 (GeneID 91603).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-418A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-418A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-418AOpen
Services
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