Bethyl Laboratories

Rabbit anti-CCDC28A Antibody Affinity Purified

Primary
Catalog #A302-392A
TargetCCDC28A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Detection of human CCDC28A by western blot of immunoprecipitates.
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Product Description

Coiled coil domain containing protein 28A (CCDC28A) contains a coiled coil domain. The gene which encodes CCDC28a is located near the locus of the pseudogene of chemokine receptor –like 1. The function of CCDC28A remains uncharacterized.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 224 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCCDC28A
Antigen Species
Human
Gene ID25901
Gene Symbol
CCDC28A
Gene Name
coiled-coil domain containing 28A
Uniprot IDQ8IWP9
Protein Name
Coiled-coil domain-containing protein 28A
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to CCDC28A immobilized on solid support.

The epitope recognized by A302-392A maps to a region between residue 224 and 274 of human coiled-coil domain containing 28A using the numbering given in entry NP_056254.1 (GeneID 25901).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A302-392A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-392A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-392AOpen
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