Bethyl Laboratories
Rabbit anti-CD28 Recombinant Monoclonal Antibody [BLR200J]
Monoclonal Antibodies
Catalog #A700-200
TargetCD28
HostRabbit
CloneBLR200J
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production, and promotion of T-cell survival [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P10747].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR200J
Host
Rabbit
Immunogen
Between 170 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetCD28
Antigen Species
Human
Gene ID940
Gene Symbol
CD28
Gene Name
CD28 molecule
Uniprot IDP10747
Protein Name
T-cell-specific surface glycoprotein CD28
Alternate Names
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 170 and the C-terminus (residue 220) of human T-cell-specific surface glycoprotein CD28 using the numbering given in entry NP_006130.1 (Gene ID 940).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-200 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-200 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-200Open
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Resources
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