Bethyl Laboratories
Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]
Monoclonal Antibodies
Catalog #A700-038
TargetCD44
HostRabbit
CloneBLR038F
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IHC-IF, IP, WB
Image 1 of 8
local_shipping
Order within 16 hours and 55 minutes for shipment by Tuesday, November 10
Product Description
CD44 is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis [taken from NCBI Entrez Gene (Gene ID: 960)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR038F
Host
Rabbit
Immunogen
Between 692 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
50 µg/ml
Target Identity
TargetCD44
Antigen Species
Human
Gene ID960
Gene Symbol
CD44
Gene Name
CD44 molecule (Indian blood group)
Uniprot IDP16070
Protein Name
CD44 antigen
Alternate Names
CD antigen CD44 • CD44 antigen • CDW44 • cell surface glycoprotein CD44 • chondroitin sulfate proteoglycan 8 • CSPG8 • ECMR-III • epican • extracellular matrix receptor III • GP90 lymphocyte homing/adhesion receptor • HCELL • hematopoietic cell E- and L-selectin ligand • heparan sulfate proteoglycan • Hermes antigen • homing function and Indian blood group system • HUTCH-I • hyaluronate receptor • IN • Indian blood group antigen • LHR • MC56 • MDU2 • MDU3 • MIC4 • Pgp1 • PGP-1 • PGP-I • phagocytic glycoprotein 1 • Phagocytic glycoprotein I • soluble CD44
Product Information
Applications
Flow Cyt, ICC, IHC, IHC-IF, IP, WB
Buffer
Phosphate Buffered Saline (PBS) with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 700 and the C-terminus (residue 742) of human CD44 using the numbering given in entry NP_000601.3 (Gene ID 960).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-038 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-038 · Lot 4 (current lot)Open
Product DatasheetPDF · A700-038 · Lot 180612Open
Safety Data SheetPDF · A700-038Open
Related Products
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request Customization
Custom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal Development
VHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH Discovery
Resources
Standard Western Blot Protocol
Read the Protocol
Immunoprecipitation Protocol
Read the Protocol
Simple Western™ Resources
Explore Simple Western™
1