Bethyl Laboratories
Rabbit anti-CD73 Recombinant Monoclonal Antibody [BLR054F]
Monoclonal Antibodies
Catalog #A700-054
TargetCD73
HostRabbit
CloneBLR054F
ReactivityHuman
ApplicationICC, IHC, IHC-IF, WB
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local_shipping
Order within 20 hours and 40 minutes for shipment by Tuesday, November 17
Product Description
CD73 is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. CD73 is used as a determinant of lymphocyte differentiation. [provided by RefSeq, Mar 2011] [taken from NCBI Entrez Gene (Gene ID: 4907)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR054F
Host
Rabbit
Immunogen
Residues 27-552 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCD73
Antigen Species
Human
Gene ID4907
Gene Symbol
NT5E
Gene Name
5'-nucleotidase ecto
Uniprot IDP21589
Protein Name
5'-nucleotidase
Alternate Names
5'-NT • 5'-nucleotidase • CALJA • CD antigen CD73 • CD73 • E5NT • ecto-5'-nucleotidase • eN • eNT • NT • NT5 • NTE • Purine 5-Prime-Nucleotidase
Product Information
Applications
ICC, IHC, IHC-IF, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human CD73 (residues 27-552, NP_002517.1).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunohistochemistry-Immunofluoresence (IHC-IF)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-054 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-054 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-054 · Lot 180924Open
Safety Data SheetPDF · A700-054Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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