Bethyl Laboratories
Rabbit anti-CD79B Recombinant Monoclonal Antibody [BLR229K]
Monoclonal Antibodies
Catalog #A700-229
TargetCD79B
HostRabbit
CloneBLR229K
ReactivityMouse, Human
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
CD79B is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P40259].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR229K
Host
Rabbit
Immunogen
Between 179 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
250 µg/ml
Target Identity
TargetCD79B
Antigen Species
Human
Gene ID974
Gene Symbol
CD79B
Gene Name
CD79b molecule
Uniprot IDP40259
Protein Name
B-cell antigen receptor complex-associated protein beta chain
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing a region between residue 200 and 250 of human B-cell antigen receptor complex-associated protein beta chain (aka CD79B) using the numbering given in entry NP_000617.1 (Gene ID 974).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 1 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-229 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-229 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-229 · Lot 220805Open
Safety Data SheetPDF · A700-229Open
Services
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