Bethyl Laboratories
Rabbit anti-CD96 Recombinant Monoclonal Antibody [BLR065G]
Monoclonal Antibodies
Catalog #A700-065
TargetCD96
HostRabbit
CloneBLR065G
ReactivityHuman
ApplicationICC, IHC, IP, WB
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Order within 20 hours and 40 minutes for shipment by Tuesday, November 17
Product Description
CD96 is an immunoglobulin superfamily member involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. CD96 promotes NK cell-target cell adhesion by interacting with PVR present on target cells. CD96 may function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P40200].
Specifications
Clonality
Recombinant Monoclonal
Clone
BLR065G
Host
Rabbit
Immunogen
Residues 29-502 (ECD)
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
1000 µg/ml
Target Identity
TargetCD96
Antigen Species
Human
Gene ID10225
Gene Symbol
CD96
Gene Name
CD96 molecule
Uniprot IDP40200
Protein Name
T-cell surface protein tactile
Product Information
Applications
ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, BSA-Free
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a recombinant protein representing the extracellular domain of human CD96 (residues 29-502, NP_005807.1).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-065 · Lot 2 (current lot)Open
Product DatasheetPDF · A700-065 · Lot 2 (current lot)Open
Safety Data SheetPDF · A700-065Open
Services
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