Bethyl Laboratories
Rabbit anti-CDC16/APC6 Antibody Affinity Purified
Primary
Catalog #A301-166A
TargetCDC16/APC6
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CDC15/APC6 is a component of the anaphase-promoting complex (APC). The APC complex functions as an ubiquitin ligase that controls cell cycle progression and promotes the transition from metaphase to anaphase. The APC complex is composed of at least 11 APC components. CDC15/APC6 amplification is associated with hepatocellular carcinoma.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 570 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCDC16/APC6
Antigen Species
Human
Gene ID8881
Gene Symbol
CDC16
Gene Name
cell division cycle 16
Uniprot IDQ13042
Protein Name
Cell division cycle protein 16 homolog
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC16/APC6 immobilized on solid support.
The epitope recognized by A301-166A maps to a region between residue 570 and 620 of human cell division cycle 16 (anaphase-promoting complex subunit 6) using the numbering given in entry NP_003894.3 (GeneID 8881).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-166A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-166A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-166AOpen
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