Bethyl Laboratories
Rabbit anti-CDC23/APC8 Antibody Affinity Purified
Primary
Catalog #A301-182A
TargetCDC23/APC8
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CDC23/APC8 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 275 and 325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCDC23/APC8
Antigen Species
Human
Gene ID8697
Gene Symbol
CDC23
Gene Name
cell division cycle 23
Uniprot IDQ9UJX2
Protein Name
Cell division cycle protein 23 homolog
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC23 immobilized on solid support.
The epitope recognized by A301-182A maps to a region between residue 275 and 325 of human cell division cycle 23 (anaphase-promoting complex subunit 8) using the numbering given in entry NP_004652.2 (GeneID 8697).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-182A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-182A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-182AOpen
Citations / Publications
Services
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