Bethyl Laboratories
Rabbit anti-CDC25c IHC Antibody Affinity Purified
Primary
Catalog #IHC-00286
TargetCDC25c
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
CDC25c (cell division cycle 25 homolog c) is a member of the Cdc25 family of phosphatases. CDC25c promotes cell cycle progression and M-phase entry by dephosphorylating CDC2/cyclin B and activating its kinase activity. CDC25c is phosphorylated and activated by multiple kinases including chk1, Cdc2/cyclin B, polo-like kinase 1, and JNK MAP kinases. The inactivation of CDC25c phosphatase activity is achieved through dephosphorylation by PP1 and PP2A phosphatases.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCDC25c
Antigen Species
Human
Gene ID995
Gene Symbol
CDC25C
Gene Name
cell division cycle 25C
Uniprot IDP30307
Protein Name
M-phase inducer phosphatase 3
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC25c immobilized on solid support.
The epitope recognized by IHC-00286 maps to a region between residue 150 and 200 of human cell division cycle 25 homolog C using the numbering given in entry NP_001781.2 (GeneID 995).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00286Open
Product DatasheetPDF · IHC-00286 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00286 · Lot 1 (current lot)Open
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