Bethyl Laboratories
Rabbit anti-CDC27 Antibody Affinity Purified
Primary
Catalog #A301-183A
TargetCDC27
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
CDC27 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions. CDC27 has been shown to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCDC27
Antigen Species
Human
Gene ID996
Gene Symbol
CDC27
Gene Name
cell division cycle 27
Uniprot IDP30260
Protein Name
Cell division cycle protein 27 homolog
Alternate Names
ANAPC3 • anaphase promoting complex subunit 3 • Anaphase-promoting complex subunit 3 • anaphase-promoting complex, protein 3 • APC3 • CDC27 homolog • CDC27Hs • cell division cycle 27 homolog • cell division cycle protein 27 homolog • D0S1430E • D0S1430E, D17S978E • D17S978E • HNUC • H-NUC • NUC2 • nuc2 homolog
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC27 immobilized on solid support.
The epitope recognized by A301-183A maps to a region between residue 225 and 275 of human cell division cycle 27 using the numbering given in entry NP_001247.2 (GeneID 996).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-183A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-183A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-183AOpen
Citations / Publications
Services
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