/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CDC27 Antibody
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Primary Antibodies  

Rabbit anti-CDC27 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (3)
Target:

CDC27

Reactivity:

Human

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

between 774 and 824

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse,

Bovine,

Rat

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Additional Product Information

CDC27 is a component of the APC/c (anaphase promoting complex/cyclosome) which is responsible for the ubiquitination and degradation of securin and cyclin B that prompts the onset of anaphase and exit from mitosis. The APC/c is composed of at least 11 subunits. Three subunits, CDC27 CDC16 and CDC23, contain a TPR (tetratricopeptide repeat) important for protein-protein interactions. CDC27 has been shown to interact with mitotic checkpoint proteins such as Mad2, p55CDC and BUBR1.

996

CDC27

cell division cycle 27

P30260

Cell division cycle protein 27 homolog

Alternate Names

ANAPC3; anaphase promoting complex subunit 3; Anaphase-promoting complex subunit 3; anaphase-promoting complex, protein 3; APC3; CDC27 homolog; CDC27Hs; cell division cycle 27 homolog; cell division cycle protein 27 homolog; D0S1430E; D0S1430E, D17S978E; D17S978E; HNUC; H-NUC; NUC2; nuc2 homolog

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.