Bethyl Laboratories
Rabbit anti-CDC40 Antibody Affinity Purified
Primary
Catalog #A303-699A
TargetCDC40
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
Cell division cycle 40 homolog (CDC40) is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression [taken from NCBI Entrez Gene (Gene ID: 51362)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCDC40
Antigen Species
Human
Gene ID51362
Gene Symbol
CDC40
Gene Name
cell division cycle 40
Uniprot IDO60508
Protein Name
Pre-mRNA-processing factor 17
Alternate Names
Product Information
Applications
IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC40 immobilized on solid support.
The epitope recognized by A303-699A maps to a region between residue 75 and 125 of human Cell Division Cycle 40 using the numbering given in entry NP_056975.1 (GeneID 51362).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A303-699A · Lot 1 (current lot)Open
Product DatasheetPDF · A303-699A · Lot 1 (current lot)Open
Safety Data SheetPDF · A303-699AOpen
Citations / Publications
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