Bethyl Laboratories
Rabbit anti-CDC5L Antibody Affinity Purified
Primary
Catalog #A301-682A
TargetCDC5L
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
CDC5L (cell division cycle 5-like protein) has been identified as a component of the human splicesome and is important to pre-mRNA splicing as well as cell cycle progression.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 752 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCDC5L
Antigen Species
Human
Gene ID988
Gene Symbol
CDC5L
Gene Name
cell division cycle 5 like
Uniprot IDQ99459
Protein Name
Cell division cycle 5-like protein
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDC5L immobilized on solid support.
The epitope recognized by A301-682A maps to a region between residue 752 and 802 of human cell division cycle 5 (CDC5) -like using the numbering given in entry NP_001244.1 (GeneID988).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-682A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-682A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-682AOpen
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