Bethyl Laboratories
Rabbit anti-CDK8 Antibody Affinity Purified
Primary
Catalog #A302-501A
TargetCDK8
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
Image 1 of 2
local_shipping
Order within 3 hours and 42 minutes for shipment by today
Product Description
Cyclin-dependent kinase 8 (CDK8) associates with cyclin C and is a component of the RNA polymerase II holoenzyme complex which phosphorylates the carboxy-terminal domain (CTD) of the largest subunit of RNA polymerase II. CDK8 has also been shown to regulate transcription by targeting the CDK7/cyclin H subunits of the general transcription initiation factor IIH (TFIIH), thus providing a link between the 'Mediator-like' protein complexes and the basal transcription machinery [taken from NCBI Entrez Gene (GeneID: 1024)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 414 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCDK8
Antigen Species
Human
Gene ID1024
Gene Symbol
CDK8
Gene Name
cyclin dependent kinase 8
Uniprot IDP49336
Protein Name
Cyclin-dependent kinase 8
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDK8 immobilized on solid support.
The epitope recognized by A302-501A maps to a region between residue 420 and 470 of human cyclin-dependent kinase 8 using the numbering given in entry NP_001251.1 (GeneID 1024).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A302-501A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-501A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-501AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1