Bethyl Laboratories
Rabbit anti-CDK9 Antibody Affinity Purified
Primary
Catalog #A303-492A
TargetCDK9
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
Cyclin-dependent kinase 9 (CDK9) is a member of the CDK family of kinases and a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. CDK9 forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCDK9
Antigen Species
Human
Gene ID1025
Gene Symbol
CDK9
Gene Name
cyclin dependent kinase 9
Uniprot IDP50750
Protein Name
Cyclin-dependent kinase 9
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CDK9 immobilized on solid support.
The epitope recognized by A303-492A maps to a region between residue 1 and 50 of human Cyclin-Dependent Kinase 9 using the numbering given in entry NP_001252.1 (GeneID 1025).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A303-492A · Lot 2 (current lot)Open
Product DatasheetPDF · A303-492A · Lot 2 (current lot)Open
Safety Data SheetPDF · A303-492AOpen
Citations / Publications
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