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Rabbit anti-CENP-F/Mitosin IHC Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Target:

CENP-F/Mitosin

Reactivity:

Human

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1700 and 1750

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (50+ slides)

10 µl (5+ slides)

$121.00 $459.00
Qty:

Product Details

Specifications
Verified Reactivity

Human

Antigen Species

Human

Concentration
250 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to CENP-F/Mitosin immobilized on solid support.

The epitope recognized by IHC-00065 maps to a region between residues 1800 and 1850 of human Centromere Protein F using the numbering given in SwissProt entry P49454 (GeneID 1063).

Antibody was affinity purified using an epitope specific to CENP-F/Mitosin immobilized on solid support.

The epitope recognized by IHC-00065-T maps to a region between residues 1800 and 1850 of human Centromere Protein F using the numbering given in SwissProt entry P49454 (GeneID 1063).

Country of Origin

USA

Additional Product Information

Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.

1063

CENPF

centromere protein F

P49454

Centromere protein F

Alternate Names

AH antigen; cell-cycle-dependent 350K nuclear protein; CENF; CENP-F; CENP-F kinetochore protein; centromere protein F; centromere protein F, 350/400kDa; CILD31; hcp-1; kinetochore protein CENPF; mitosin; PRO1779; STROMS

Applications

Acetone-Fixed Cells 1:100 - 1:500:

Formaldehyde or methanol fixation is not recommended.