Bethyl Laboratories
Rabbit anti-CENP-F/Mitosin IHC Antibody Affinity Purified
Primary
Catalog #IHC-00315
TargetCENP-F/Mitosin
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
Centromere protein F (CENP-F) is a microtubule binding protein and component of the kinetochore. It is essential for kinetochore attachment, chromosome alignment, and the spindle checkpoint. CENP-F appears to be important to the association of the NudE-related proteins Ndel1 and Nde1 to the Lis1/dynein motor complex and their role in kinetochore function. CENP-F is a farnesylated protein and the farnesylation of CENP-F appears to be central to its role in mediating kinetochore-microtubule interactions as well as its degradation after mitosis. Recently, CENP-F expression has been correlated with markers of poor outcome in breast cancer.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2275 and 2325
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetCENP-F/Mitosin
Antigen Species
Human
Gene ID1063
Gene Symbol
CENPF
Gene Name
centromere protein F
Uniprot IDP49454
Protein Name
Centromere protein F
Alternate Names
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CENP-F/Mitosin immobilized on solid support.
The epitope recognized by IHC-00315 maps to a region between residue 2350 and 2400 of human centromere protein F (Mitosin) using the numbering given in entry AAA82889.1 (GeneID 1063).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00315Open
Product DatasheetPDF · IHC-00315 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00315 · Lot 1 (current lot)Open
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