Bethyl Laboratories
Rabbit anti-CENP-T Antibody Affinity Purified
Primary
Catalog #A302-314A
TargetCENP-T
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 7 hours and 24 minutes for shipment by Tuesday, November 17
Product Description
Centromere protein T (CENP-T) is a component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. CENP-T is one of the basic inner kinetochore proteins with most further proteins binding downstream, suggesting a fundamental role of CENP-T in kinetochore function [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q96BT3].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 511 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCENP-T
Antigen Species
Human
Gene ID80152
Gene Symbol
CENPT
Gene Name
centromere protein T
Uniprot IDQ96BT3
Protein Name
Centromere protein T
Alternate Names
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CENP-T immobilized on solid support.
The epitope recognized by A302-314A maps to a region between residue 520 and 561 of human centromere protein T using the numbering given in entry NP_079358.3 (GeneID 80152).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-314A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-314A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-314AOpen
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1