Bethyl Laboratories
Rabbit anti-CEP131/AZ1 Antibody Affinity Purified
Primary
Catalog #A301-416A
TargetCEP131/AZ1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 350 and 400
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCEP131/AZ1
Antigen Species
Human
Gene ID22994
Gene Symbol
CEP131
Gene Name
centrosomal protein 131
Uniprot IDQ9UPN4
Protein Name
Centrosomal protein of 131 kDa
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CEP131/AZ1 immobilized on solid support.
The epitope recognized by A301-416A maps to a region between residue 325 and 375 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-416A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-416A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-416AOpen
Services
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