Bethyl Laboratories
Rabbit anti-CEP131/AZ1 Antibody Affinity Purified
Primary
Catalog #A301-417A
TargetCEP131/AZ1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF, IP, WB
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Product Description
CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1033 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCEP131/AZ1
Antigen Species
Human
Gene ID22994
Gene Symbol
CEP131
Gene Name
centrosomal protein 131
Uniprot IDQ9UPN4
Protein Name
Centrosomal protein of 131 kDa
Product Information
Applications
ICC-IF, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CEP131/AZ1 immobilized on solid support.
The epitope recognized by A301-417A maps to a region between residue 1033 and 1083 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-417A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-417A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-417AOpen
Citations / Publications
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