Bethyl Laboratories

Rabbit anti-CEP131/AZ1 IHC Antibody Affinity Purified

Primary
Catalog #IHC-00363
TargetCEP131/AZ1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
Detection of human CEP131/AZ1 by immunocytochemistry.
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Product Description

CEP131/AZ1 was originally identified as a transcript that was increased in cells exposed to the demethylating agent, azacytidine. It was found to localize to the pre-acrosome region of spermatids and therefore thought to play a role in spermatogenesis. CEP131/AZ1 has also been identified as a component of the centrosome.

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1033 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
Antigen Species
Human
Gene ID22994
Gene Symbol
CEP131
Gene Name
centrosomal protein 131
Uniprot IDQ9UPN4
Protein Name
Centrosomal protein of 131 kDa
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to CEP131/AZ1 immobilized on solid support.

The epitope recognized by IHC-00363 maps to a region between residue 1033 and 1083 of human centrosomal protein 131 kDa (5-azacytidine induced 1) using the numbering given in entry EAW89632.1 (GeneID 22994).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

Formaldehyde-Fixed Cells 1:100 - 1:500:

Permeabilization with Triton-X 100 is recommended.

Acetone-Fixed Cells 1:100 - 1:500.

Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500

Documents

Safety Data SheetPDF · IHC-00363Open
Product DatasheetPDF · IHC-00363 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00363 · Lot 1 (current lot)Open
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