Bethyl Laboratories
Rabbit anti-CEP290 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00365
TargetCEP290
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationICC-IF
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Product Description
Centrosomal protein 290 (CEP290) is a member of the nephrocystins, a family of proteins involved in renal cystic diseases. CEP290 and the nephrocystins appear to play a role in cilia and basal body function. Loss-of-function mutations in CEP290 result in Joubert syndrome and related disorders characterized by brain malformations, developmental delay, ataxia, irregular breathing, renal disease, and hepatic fibrosis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 2429 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
500 µg/ml
Target Identity
TargetCEP290
Antigen Species
Human
Gene ID80184
Gene Symbol
CEP290
Gene Name
centrosomal protein 290
Uniprot IDO15078
Protein Name
Centrosomal protein of 290 kDa
Alternate Names
3H11Ag • Bardet-Biedl syndrome 14 protein • BBS14 • cancer/testis antigen 87 • centrosomal protein 290kDa • centrosomal protein of 290 kDa • Cep290 • CT87 • CTCL tumor antigen se2-2 • JBTS5 • LCA10 • Meckel syndrome, type 4 • MKS4 • monoclonal antibody 3H11 antigen • Nephrocystin-6 • nephrocytsin-6 • NPHP6 • POC3 • POC3 centriolar protein homolog • prostate cancer antigen T21 • rd16 • SLSN6 • tumor antigen se2-2
Product Information
Applications
ICC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CEP290 immobilized on solid support.
The epitope recognized by IHC-00365 maps to a region between residue 2429 and 2479 of human centrosomal protein 290kDa using the numbering given in entry NP_079390.3 (GeneID 80184).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Formaldehyde-Fixed Cells 1:100 - 1:500:
Permeabilization with Triton-X 100 is recommended.
Acetone fixation is not recommended.
Immunocytochemistry-Immunofluoresence (ICC-IF)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00365Open
Product DatasheetPDF · IHC-00365 · Lot 2 (current lot)Open
Product DatasheetPDF · IHC-00365 · Lot 2 (current lot)Open
Citations / Publications
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Resources
Standard Western Blot Protocol
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