Bethyl Laboratories
Rabbit anti-CEP41 Antibody Affinity Purified
Primary
Catalog #A301-798A
TargetCEP41
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP, WB
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Product Description
CEP41 (centrosomal protein of 41 kDa) is the protein product of the testis-specific gene A14 (TSGA14). TSGA14 was originally identified as a gene adjacent to the imprinted gene MEST. Studies of TSGA14 revealed that unlike MEST, TSGA14 is not an imprinted gene. CEP41 was also identified as a true centrosomal protein in a mass-spectometry-based proteomic analysis of human centrosomes. The role of CEP41 in the centrosome remains uncharacterized.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 150 and 200
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCEP41
Antigen Species
Human
Gene ID95681
Gene Symbol
CEP41
Gene Name
centrosomal protein 41
Uniprot IDQ9BYV8
Protein Name
Centrosomal protein of 41 kDa
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CEP41 immobilized on solid support.
The epitope recognized by A301-798A maps to a region between residue 150 and 200 of human centrosomal protein 41kDa using the numbering given in entry NP_061188.1 (GeneID 95681).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-798A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-798A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-798AOpen
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