Bethyl Laboratories
Rabbit anti-ch-TOG Antibody Affinity Purified
Primary
Catalog #A301-970A
Targetch-TOG
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIP, WB
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Product Description
Colonic and hepatic tumor over-expressed protein (ch-TOG) is a protein originally described as a gene over-expressed in human hepatomas and colonic tumors. It was found to be a homolog of the xenopus egg protein, XMAP214, a microtubule associated protein (MAP). The human ch-TOG protein has been shown to promote microtubule assembly and is a major component of microtubule asters. It is required for centrosome integrity, spindle pole organization and bipolar spindle assembly.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1925 and 1975
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
Targetch-TOG
Antigen Species
Human
Gene ID9793
Gene Symbol
CKAP5
Gene Name
cytoskeleton associated protein 5
Uniprot IDQ14008
Protein Name
Cytoskeleton-associated protein 5
Product Information
Applications
IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to ch-TOG immobilized on solid support.
The epitope recognized by A301-970A maps to a region between residue 1425 and 1475 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000 (3-8% Tris-acetate gel recommended)
Documents
Product DatasheetPDF · A301-970A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-970A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-970AOpen
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