/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-ch-TOG Antibody
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Primary Antibodies  

Rabbit anti-ch-TOG Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (1)
Target:

ch-TOG

Reactivity:

Human

,

Mouse

Applications:

IP

,

WB

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 1982 and C-terminus

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Mouse,

Human

Antigen Species

Human

Concentration
200 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to ch-TOG immobilized on solid support.

The epitope recognized by A301-971A maps to a region between residue 1982 and 2032 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to ch-TOG immobilized on solid support.

The epitope recognized by A301-971A-T maps to a region between residue 1982 and 2032 of human colonic and hepatic tumor over-expressed protein using the numbering given in entry NP_001008938.1 (GeneID 9793).

Country of Origin

USA

Additional Product Information

Colonic and hepatic tumor over-expressed protein (ch-TOG) is a protein originally described as a gene over-expressed in human hepatomas and colonic tumors. It was found to be a homolog of the xenopus egg protein, XMAP214, a microtubule associated protein (MAP). The human ch-TOG protein has been shown to promote microtubule assembly and is a major component of microtubule asters. It is required for centrosome integrity, spindle pole organization and bipolar spindle assembly.

9793

CKAP5

cytoskeleton associated protein 5

Q14008

Cytoskeleton-associated protein 5

Alternate Names

CHTOG; ch-TOG; colonic and hepatic tumor overexpressed gene protein; colonic and hepatic tumor over-expressed gene protein; cytoskeleton-associated protein 5; MSPS; TOG; TOGp

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.