Bethyl Laboratories
Rabbit anti-Chk1 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00004
TargetChk1
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Chk1 functions as a checkpoint protein kinase that is critical to the regulation of the Cdc25a and Cdc25c phosphatases that promote S and G2/M phase progression, respectively. Chk1 kinase activity inhibits cell cycle progression in the presence of DNA damage by phosphorylating Cdc25a and Cdc25c and causing the degradation of Cdc25a and cytoplasmic sequestration of Cdc25c.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 250 and 300
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetChk1
Antigen Species
Human
Gene ID1111
Gene Symbol
CHEK1
Gene Name
checkpoint kinase 1
Uniprot IDO14757
Protein Name
Serine/threonine-protein kinase Chk1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Chk1 immobilized on solid support.
The epitope recognized by IHC-00004 maps to a region between residue 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00004Open
Product DatasheetPDF · IHC-00004 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00004 · Lot 1 (current lot)Open
Citations / Publications
Services
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