Bethyl Laboratories

Rabbit anti-CIAPIN1 Antibody Affinity Purified

Primary
Catalog #A302-808A
TargetCIAPIN1
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
Detection of human CIAPIN1 by western blot of immunoprecipitates.
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Product Description

CIAPIN1 (cytokine-induced apoptosis inhibitor 1) is also known as Anamorsin. CIAPIN1 may be required for the maturation of extramitochondrial Fe/S proteins. CIAPIN1 has anti-apoptotic effects in the cell, and is involved in negative control of cell death upon cytokine withdrawal. CIAPIN1 also promotes development of hematopoietic cells [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ Q6FI81].

Specifications

Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 125 and 175
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCIAPIN1
Antigen Species
Human
Gene ID57019
Gene Symbol
CIAPIN1
Gene Name
cytokine induced apoptosis inhibitor 1
Uniprot IDQ6FI81
Protein Name
Anamorsin
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA

Production & Epitope

Antibody was affinity purified using an epitope specific to CIAPIN1 immobilized on solid support.

The epitope recognized by A302-808A maps to a region between residue 100 and 150 of human Cytokine Induced Apoptosis Inhibitor 1 using the numbering given in entry NP_064709.2 (GeneID 57019).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.

Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate

Documents

Product DatasheetPDF · A302-808A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-808A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-808AOpen
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