Bethyl Laboratories
Rabbit anti-CIP2A Antibody Affinity Purified
Primary
Catalog #A301-453A
TargetCIP2A
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP
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Product Description
CIP2A (cancerous inhibitor of PP2A) was originally identified as p90, a cytoplasmic autoantigen found in the serum from a cancer patient. It was later described as a protein that interacted with the c-myc oncogene and functioned as an inhibitor of protein phosphatase 2A (PP2A) activity. Inhibition of PP2A phosphatase activity by CIP2A prevents c-myc phosphorylation and counteracts c-myc proteolytic degradation, thereby stabilizing c-myc and promoting cell growth and transformation. CIP2A is found to be overexpressed in some human malignancies and is thus classified as an oncogene that may be an important contributor to tumor formation.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 575 and 625
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCIP2A
Antigen Species
Human
Gene ID57650
Gene Symbol
CIP2A
Gene Name
KIAA1524
Uniprot IDQ8TCG1
Protein Name
Protein CIP2A
Alternate Names
Product Information
Applications
IHC, IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CIP2A immobilized on solid support.
The epitope recognized by A301-453A maps to a region between residue 550 and 600 of human cancerous inhibitor of PP2A using the numbering given in entry NP_065941.1 (GeneID 57650).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-453A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-453A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-453AOpen
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