Bethyl Laboratories
Rabbit anti-CK7 Recombinant Monoclonal Antibody [BC1]
Monoclonal Antibodies
Catalog #A700-186
TargetCK7
HostRabbit
CloneBC1
ReactivityHuman
ApplicationFlow Cyt, ICC, IHC, IP, WB
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Product Description
Cytokeratin 7 is a type II member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. CK7 is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. [taken from NCBI Entrez Gene (Gene ID: 3855)].
Specifications
Clonality
Recombinant Monoclonal
Clone
BC1
Host
Rabbit
Immunogen
C-terminal peptide
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Purified
Concentration
100 µg/ml
Target Identity
TargetCK7
Antigen Species
Human
Gene ID3855
Gene Symbol
KRT7
Gene Name
keratin 7
Uniprot IDP08729
Protein Name
Keratin, type II cytoskeletal 7
Product Information
Applications
Flow Cyt, ICC, IHC, IP, WB
Buffer
Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Recombinant antibody was purified from cell culture supernatant.
Immunogen was a peptide representing the C-terminus of human cytoskeletal keratin 7 (NP_005547.3, Gene ID 3855).
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Flow Cytometry (Flow Cyt)Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells.
Immunocytochemistry (ICC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Immunohistochemistry (IHC)1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)20 µl/mg lysate
Western Blot (WB)1:1000
Documents
Product DatasheetPDF · A700-186 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-186 · Lot 1 (current lot)Open
Product DatasheetPDF · A700-186 · Lot 1 (current lot)Open
Safety Data SheetPDF · A700-186Open
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
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Full custom monoclonal development, from immunization through validation.
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Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
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