Bethyl Laboratories
Rabbit anti-CLOCK Antibody Affinity Purified
Primary
Catalog #A302-617A
TargetCLOCK
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CLOCK (circadian locomotor output cycles protein kaput) belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1 [taken from NCBI Entrez Gene (GeneID: 9575)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 450 and 500
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCLOCK
Antigen Species
Human
Gene ID9575
Gene Symbol
CLOCK
Gene Name
clock circadian regulator
Uniprot IDO15516
Protein Name
Circadian locomoter output cycles protein kaput
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CLOCK immobilized on solid support.
The epitope recognized by A302-617A maps to a region between residue 450 and 500 of human circadian locomoter output cycles kaput protein using the numbering given in entry NP_004889.1 (GeneID 9575).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-617A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-617A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-617AOpen
Citations / Publications
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