Bethyl Laboratories
Rabbit anti-CNOT3 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00623
TargetCNOT3
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IHC-IF
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Product Description
CNOT3 (CCR4-NOT transcription complex subunit 3) is a component of the CCR4-NOT core complex that functions as a general transcriptional regulator. In a genome-wide siRNA screen for genes essential for mouse embryonic stem cell self-renewal, CNOT3 was identified and found to co-occupy gene promoters with c-Myc and Zfx.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 525 and 575
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCNOT3
Antigen Species
Human
Gene ID4849
Gene Symbol
CNOT3
Gene Name
CCR4-NOT transcription complex subunit 3
Uniprot IDO75175
Protein Name
CCR4-NOT transcription complex subunit 3
Product Information
Applications
IHC, IHC-IF
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CNOT3 immobilized on solid support.
The epitope recognized by IHC-00623 maps to a region between residue 525 and 575 of human CCR4-NOT transcription complex, subunit 3 using the numbering given in entry NP_055331.1 (GeneID 4849).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Immunohistochemistry-Immunofluoresence (IHC-IF)1:50 - 1:500
Documents
Safety Data SheetPDF · IHC-00623Open
Product DatasheetPDF · IHC-00623 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00623 · Lot 1 (current lot)Open
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