Rabbit anti-Coronin 1 Antibody Affinity Purified

Specifications

Production & Epitope

Antibody was affinity purified using an epitope specific to Coronin 1 immobilized on solid support.

The epitope recognized by A300-932A maps to a region between residue 410 and the C-terminus (residue 461) of human Coronin 1 (Coronin, Actin Binding Protein 1A) using the numbering given in entry NP_009005.1 (GeneID 11151).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).