Bethyl Laboratories
Rabbit anti-COX4 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00546
TargetCOX4
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
COX4 (cytochrome c oxidase, subunit IV isoform 1) is one of 13 subunits of the multiprotein COX (cytochrome oxidase) complex which functions as the terminal enzyme in the mitochondrial respiratory chain.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 25 and 75
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
100 µg/ml
Target Identity
TargetCOX4
Antigen Species
Human
Gene ID1327
Gene Symbol
COX4I1
Gene Name
cytochrome c oxidase subunit 4I1
Uniprot IDP13073
Protein Name
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to COX4 immobilized on solid support.
The epitope recognized by IHC-00546 maps to a region between residue 25 and 75 of human cytochrome c oxidase subunit IV isoform 1 using the numbering given in entry NP_001852.1 (GeneID 1327).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00546Open
Product DatasheetPDF · IHC-00546 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00546 · Lot 1 (current lot)Open
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