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Rabbit anti-CPSF100 Antibody Affinity Purified

bethyl
Bethyl Laboratories Catalog #
Validated
Target:
CPSF100
Reactivity:
Human, Mouse
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
Between 475 and 525
Isotype:
IgG
Conjugate:
Unconjugated
Purity:
Antigen Affinity Purified
100 µl (200 µg/ml)

10 µl (200 µg/ml)

$121.00 $433.00
Qty:

Product Details

Specifications

Verified Reactivity
Mouse, Human
Presumed Reactivity
Bovine
Antigen Species
Human
Concentration
200 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
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Country of Origin
USA

Additional Product Information

CPSF100/CPSF2 (cleavage and polyadenylation specific factor 2, 100 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3’-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. CPSF100/CPSF2 and CPSF73/CPSF3 are both members of the metallo-beta-lactamase superfamily of zinc-dependent hydrolases.

53981
CPSF2
cleavage and polyadenylation specific factor 2
Q9P2I0
Cleavage and polyadenylation specificity factor subunit 2

Alternate Names

cleavage and polyadenylation specific factor 2, 100kDa; cleavage and polyadenylation specificity factor 100 kDa subunit; cleavage and polyadenylation specificity factor subunit 2; CPSF 100 kDa subunit; CPSF 100kDa subunit; CPSF100

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (Cat. No. A120-101P).

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.