Bethyl Laboratories
Rabbit anti-CPSF100 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00551
TargetCPSF100
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
CPSF100/CPSF2 (cleavage and polyadenylation specific factor 2, 100 kDa subunit) is a subunit of the cleavage and polyadenylation specificity factor (CPSF) involved in mammalian mRNA 3’-end processing. CPSF, along with CstF (cleavage and stimulation factor), is critical for the recognition of cis-acting sequence elements on the pre-mRNA that dictate the site of cleavage and subsequent polyadenylation. CPSF exists as a complex of four proteins that includes CPSF160/CPSF1, CPSF100/CPSF2, CPSF73/CPSF3, and CPSF30/CPSF4. CPSF100/CPSF2 and CPSF73/CPSF3 are both members of the metallo-beta-lactamase superfamily of zinc-dependent hydrolases.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 475 and 525
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCPSF100
Antigen Species
Human
Gene ID53981
Gene Symbol
CPSF2
Gene Name
cleavage and polyadenylation specific factor 2
Uniprot IDQ9P2I0
Protein Name
Cleavage and polyadenylation specificity factor subunit 2
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CPSF100 immobilized on solid support.
The epitope recognized by IHC-00551 maps to a region between residue 450 and 500 of human cleavage and polyadenylation specific factor 2, 100kDa using the numbering given in entry NP_059133.1 (GeneID 53981).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00551Open
Product DatasheetPDF · IHC-00551 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00551 · Lot 1 (current lot)Open
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