/ ANTIBODIES/ANTISERA / Primary Antibodies / Rabbit anti-CPSF59 Antibody
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Primary Antibodies  

Rabbit anti-CPSF59 Antibody Affinity Purified

bethyl
Bethyl Laboratories

Catalog #

Validated
Citations (4)
Target:

CPSF59

Reactivity:

Human

Applications:

IHC

,

IP

Host:

Rabbit

Clonality:

Polyclonal

Format:

Whole IgG

Immunogen:

Between 175 and 225

Isotype:

IgG

Conjugate:

Unconjugated

Purity:

Antigen Affinity Purified

100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

$121.00 $433.00
Qty:
Bulk Request

Product Details

Specifications
Verified Reactivity

Human

Presumed Reactivity

Mouse

Antigen Species

Human

Concentration
1000 µg/ml
Storage

2 - 8 °C

Shelf Life

1 year from date of receipt

Physical State
Liquid
Buffer

Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide


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Production Procedures

Antibody was affinity purified using an epitope specific to CPSF59 immobilized on solid support.

The epitope recognized by A301-360A-T maps to a region between residue 175 and 225 of human cleavage and polyadenylation specificity factor 7, 59 kDa subunit using the numbering given in entry NP_079087.2 (GeneID 79869).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Antibody was affinity purified using an epitope specific to CPSF59 immobilized on solid support.

The epitope recognized by A301-360A maps to a region between residue 175 and 225 of human cleavage and polyadenylation specificity factor 7, 59 kDa subunit using the numbering given in entry NP_079087.2 (GeneID 79869).

Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.

Country of Origin

USA

Additional Product Information

CPSF59 is the the 59 kDa subunit of the cleavage factor Im complex (CFIm). The CFIm is a heterodimer composed of a 25 kDa (CPSF5) subunit and either the 59 kDa (CPSF7) or 68 kDa (CPSF6) subunit and is essential to the first step of pre-mRNA 3’-end processing. CPSF59 bears an RNA-recognition motif (RRM).

79869

CPSF7

cleavage and polyadenylation specific factor 7

Q8N684

Cleavage and polyadenylation specificity factor subunit 7

Alternate Names

CFIm59; cleavage and polyadenylation specificity factor 59 kDa subunit; cleavage and polyadenylation specificity factor subunit 7; cleavage factor Im complex 59 kDa subunit; CPSF 59 kDa subunit; pre-mRNA cleavage factor I, 59 kDa subunit; pre-mRNA cleavage factor Im 59 kDa subunit

Applications

All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.

Western blots of immunoprecipitates are performed using Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.