Bethyl Laboratories
Rabbit anti-CPSF68 Antibody Affinity Purified
Primary
Catalog #A301-356A
TargetCPSF68
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC, IP, WB
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Product Description
Cleavage and polyadenylation specific factor 68 (CPSF68) is the 68 kDa subunit of the cleavage factor Im complex (CFIm). The CFIm is a heterodimer composed of a 25 kDa (CPSF5) subunit and either the 59 kDa (CPSF7) or 68 kDa (CPSF6) subunit and is essential to the first step of pre-mRNA 3’-end processing. CPSF68 bears an N-terminal RNA- recognition motif (RRM) that is necessary for binding CPS5.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCPSF68
Antigen Species
Human
Gene ID11052
Gene Symbol
CPSF6
Gene Name
cleavage and polyadenylation specific factor 6
Uniprot IDQ16630
Protein Name
Cleavage and polyadenylation specificity factor subunit 6
Alternate Names
CFIM • CFIM68 • CFIM72 • cleavage and polyadenylation specific factor 6, 68kDa • cleavage and polyadenylation specificity factor 68 kDa subunit • cleavage and polyadenylation specificity factor subunit 6 • cleavage factor Im complex 68 kDa subunit • CPSF 68 kDa subunit • HPBRII-4 • HPBRII-7 • pre-mRNA cleavage factor I, 68kD subunit • pre-mRNA cleavage factor Im (68kD) • pre-mRNA cleavage factor Im 68 kDa subunit • protein HPBRII-4/7
Product Information
Applications
IHC, IP, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CPSF68 immobilized on solid support.
The epitope recognized by A301-356A maps to a region between residue 1 and 50 of human cleavage and polyadenylation specific factor 6, 68kDa using the numbering given in entry NP_008938.1 (GeneID 11052).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-356A · Lot 3 (current lot)Open
Product DatasheetPDF · A301-356A · Lot 3 (current lot)Open
Safety Data SheetPDF · A301-356AOpen
Citations / Publications
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