Bethyl Laboratories
Rabbit anti-CRABP2 Antibody Affinity Purified
Primary
Catalog #A300-809A
TargetCRABP2
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, WB
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Product Description
Cellular retinoic acid binding protein 2 (CRABP2) is a coactivator present in the retinoic acid (RA)-dependent nuclear complex. CRABP2 has been shown to mediate retinoic acid activity in skin to influence skin cell growth and differentiation. It has also been shown to mediate the growth suppressive effects of retinoic acid in breast cancer. CRABP2’s role in cell differentiation may be linked to control of the cell-cycle since it has been identified as an interacting partner of cyclin D3 in CRABPII and RAR ternary complex.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 88 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
200 µg/ml
Target Identity
TargetCRABP2
Antigen Species
Human
Gene ID1382
Gene Symbol
CRABP2
Gene Name
cellular retinoic acid binding protein 2
Uniprot IDP29373
Protein Name
Cellular retinoic acid-binding protein 2
Alternate Names
Product Information
Applications
IHC, WB
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CRABP2 immobilized on solid support.
The epitope recognized by A300-809A maps to a region between residue 80 and the C-terminus (residue 138) of human Cellular Retinoic Acid Binding Protein 2 using the numbering given in entry NP_001869.1 (GeneID 1382).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Immunohistochemistry (IHC)1:200 - 1:1000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A300-809A · Lot 1 (current lot)Open
Product DatasheetPDF · A300-809A · Lot 1 (current lot)Open
Safety Data SheetPDF · A300-809AOpen
Citations / Publications
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Resources
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