Bethyl Laboratories
Rabbit anti-CROP/Luc7A IHC Antibody Affinity Purified
Primary
Catalog #IHC-00600
TargetCROP/Luc7A
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationIHC
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Product Description
Cisplatin resistance-associated overexpressed protein (CROP) was originally cloned by differential display from cisplatin-resistant cells. It is an SR-related protein and the human homolog of the yeast protein, Luc7p. Luc7p is an essential protein involved in vegetative growth and 5’-splice recognition. CROP/Luc7a contains arginine- and glutamate-rich domains (RE domains) and arginine- and serine-rich domains (RS domains). It localizes to the nucleus and is distributed as speckles. CROP/Luc7a has been shown to interact with SF2/ASF, a protein involved in RNA splicing. Due to this association and the known function of its yeast homolog, CROP/Luc7a is proposed to play a role in RNA splicing.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCROP/Luc7A
Antigen Species
Human
Gene ID51747
Gene Symbol
LUC7L3
Gene Name
LUC7 like 3 pre-mRNA splicing factor
Uniprot IDO95232
Protein Name
Luc7-like protein 3
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CROP/Luc7A immobilized on solid support.
The epitope recognized by IHC-00600 maps to a region between residue 1 and 50 of human cisplatin resistance-associated overexpressed protein using the numbering given in entry NP_057508.2 (GeneID 51747).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00600Open
Product DatasheetPDF · IHC-00600 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00600 · Lot 1 (current lot)Open
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