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Rabbit anti-CRSP3 Antibody

Bethyl Laboratories®

Bethyl Laboratories® Catalog #

Validated
Target:
CRSP3
Reactivity:
Human
Host:
Rabbit
Clonality:
Polyclonal
Format:
Whole IgG
Immunogen:
between 400 and 450
IsoType:
IgG
Purity:
Antigen Affinity Purified
100 µl (1000 µg/ml)

10 µl (1000 µg/ml)

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Product Details

Specifications
Verified Reactivity
Human
Antigen Species
Human
Stock Concentration
1000 µg/ml
Storage
2 - 8 °C
Shelf Life
1 year from date of receipt
Physical State
Liquid
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
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Prod Procedures
Antibody was affinity purified using an epitope specific to CRSP3 immobilized on solid support.

The epitope recognized by A300-424A-T maps to a region between residues 400 and 450 of human Cofactor Required for Sp1 transcriptional activation, subunit 3 using the numbering given in Swiss-Prot entry Q9ULK4 (GeneID 9439).

Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

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Gene & Protein Information

CRSP3 is a subunit of the CRSP (cofactor required for SP1 activation) complex required for efficient transcriptional activation by the SP1 transcription factor. It has also been identified as a component of the mediator complex that bridges nuclear hormone receptors to RNA polymerase II. CRSP3 may also function as a metastasis suppressor through its transcriptional regulation of the metastasis suppressor, KiSS-1.

9439

MED23

mediator complex subunit 23

Q9ULK4

Mediator of RNA polymerase II transcription subuni

Alternate Names

130 kDa transcriptional co-activator, 133 kDa transcriptional co-activator, activator-recruited cofactor 130 kDa component, ARC130, cofactor required for Sp1 transcriptional activation subunit 3, CRSP complex subunit 3, CRSP130, CRSP133, CRSP3, DRIP130, hSur-2, Mediator complex subunit 23, mediator of RNA polymerase II transcription subunit 23, MRT18, Protein sur-2 homolog, SUR2, SUR-2, Transcriptional coactivator CRSP130, vitamin D3 receptor interacting protein, Vitamin D3 receptor-interacting protein complex 130 kDa component

Applications

Western blot of immunoprecipitates performed using Normal Pig Serum (Cat. No. S100-020), Goat anti-Rabbit Light Chain HRP Conjugate (Cat. No. A120-113P) and 4-8% SDS-PAGE.