Bethyl Laboratories
Rabbit anti-CRSP7 Antibody Affinity Purified
Primary
Catalog #A302-370A
TargetCRSP7
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
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Product Description
CRSP7 (cofactor required for Sp1 transcriptional activation, subunit 7) is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes such as thyroid hormone receptor-(TR-) associated protein (TRAP) and mediator complexes. CRSP7 is also known as MED26 (mediator complex subunit 26 [taken from NCBI Entrez Gene (GeneID: 9441)].
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 1 and 50
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCRSP7
Antigen Species
Human
Gene ID9441
Gene Symbol
MED26
Gene Name
mediator complex subunit 26
Uniprot IDO95402
Protein Name
Mediator of RNA polymerase II transcription subunit 26
Alternate Names
activator-recruited cofactor 70 kDa component • ARC70 • cofactor required for Sp1 transcriptional activation subunit 7 • cofactor required for Sp1 transcriptional activation, subunit 7 (70kD) • cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa • CRSP complex subunit 7 • CRSP7 • CRSP70 • Mediator complex subunit 26 • mediator of RNA polymerase II transcription subunit 26 • transcriptional coactivator CRSP70
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CRSP7 immobilized on solid support.
The epitope recognized by A302-370A maps to a region between residue 1 and 50 of human cofactor required for Sp1 transcriptional activation, subunit 7 using the numbering given in entry NP_004822.2 (GeneID 9441).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A302-370A · Lot 1 (current lot)Open
Product DatasheetPDF · A302-370A · Lot 1 (current lot)Open
Safety Data SheetPDF · A302-370AOpen
Citations / Publications
Services
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