Bethyl Laboratories
Rabbit anti-CSTF2T/TauCSTF64 Antibody Affinity Purified
Primary
Catalog #A301-486A
TargetCSTF2T/TauCSTF64
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIP
Image 1 of 1
local_shipping
Order within 17 hours and 20 minutes for shipment by Tuesday, November 10
Product Description
CSTF2T, also known as tauCSTF-64, is a paralog of CstF-64, a component of the cleavage stimulation factor (CstF) that processes 3’-end formation and polyadenylation of mRNAs. The human CSTF2T/TauCSTF64 gene is located at 10q22-23, a site associated with neurological problems and male infertility. CSTF2T is expressed in all tissues and throughout meiosis in male germ cells. CstF-64 is also expressed ubiquitously; however because it is located on the X chromosome, its expression is temporarily silenced in germ cells by meiotic sex chromosome inactivation. During this inactivation, it is hypothesized that CSTF2T/TauCSTF64 can compensate for the loss of CstfF-64 expression. Recently, targeted disruption of CSTF2T/TauCSTF64 in mice has been shown to cause aberrant spermatogenesis and infertility providing more evidence for its importance in spermatogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 225 and 275
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCSTF2T/TauCSTF64
Antigen Species
Human
Gene ID23283
Gene Symbol
CSTF2T
Gene Name
cleavage stimulation factor subunit 2 tau variant
Uniprot IDQ9H0L4
Protein Name
Cleavage stimulation factor subunit 2 tau variant
Alternate Names
CF-1 64 kDa subunit tau • CF-1 64 kDa subunit tau variant • cleavage stimulation factor 64 kDa subunit tau • Cleavage stimulation factor 64 kDa subunit tau variant • cleavage stimulation factor subunit 2 tau variant • cleavage stimulation factor subunit 2, tau • cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau • cleavage stimulation factor, 3' pre-RNA, subunit 2, tau • CSTF 64 kDa subunit tau • CSTF 64 kDa subunit tau variant • CstF-64T • tauCstF-64
Product Information
Applications
IP
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CSTF2T/TauCSTF64 immobilized on solid support.
The epitope recognized by A301-486A maps to a region between residue 225 and 275 of human cleavage stimulation factor 64 kDa subunit, tau variant using the numbering given in entry NP_056050.1 (GeneID 23283).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunoprecipitation (IP)6 µg/mg lysate
Documents
Product DatasheetPDF · A301-486A · Lot 1 (current lot)Open
Product DatasheetPDF · A301-486A · Lot 1 (current lot)Open
Safety Data SheetPDF · A301-486AOpen
Citations / Publications
Services
Antibody Conjugation
Antibody and conjugate formats developed together, built to your specification.
Request CustomizationCustom Monoclonal Antibody Discovery Services
Full custom monoclonal development, from immunization through validation.
Explore Monoclonal DevelopmentVHH Discovery
Single-domain antibodies for structurally difficult targets a conventional monoclonal can't reach.
Explore VHH DiscoveryResources
1