Bethyl Laboratories
Rabbit anti-CSTF2T/TauCSTF64 Antibody Affinity Purified
Primary
Catalog #A301-487A
TargetCSTF2T/TauCSTF64
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC, IP, WB
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Product Description
CSTF2T, also known as tauCSTF-64, is a paralog of CstF-64, a component of the cleavage stimulation factor (CstF) that processes 3’-end formation and polyadenylation of mRNAs. The human CSTF2T/TauCSTF64 gene is located at 10q22-23, a site associated with neurological problems and male infertility. CSTF2T is expressed in all tissues and throughout meiosis in male germ cells. CstF-64 is also expressed ubiquitously; however because it is located on the X chromosome, its expression is temporarily silenced in germ cells by meiotic sex chromosome inactivation. During this inactivation, it is hypothesized that CSTF2T/TauCSTF64 can compensate for the loss of CstfF-64 expression. Recently, targeted disruption of CSTF2T/TauCSTF64 in mice has been shown to cause aberrant spermatogenesis and infertility providing more evidence for its importance in spermatogenesis.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 566 and C-terminus
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCSTF2T/TauCSTF64
Antigen Species
Human
Gene ID23283
Gene Symbol
CSTF2T
Gene Name
cleavage stimulation factor subunit 2 tau variant
Uniprot IDQ9H0L4
Protein Name
Cleavage stimulation factor subunit 2 tau variant
Alternate Names
CF-1 64 kDa subunit tau • CF-1 64 kDa subunit tau variant • cleavage stimulation factor 64 kDa subunit tau • Cleavage stimulation factor 64 kDa subunit tau variant • cleavage stimulation factor subunit 2 tau variant • cleavage stimulation factor subunit 2, tau • cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau • cleavage stimulation factor, 3' pre-RNA, subunit 2, tau • CSTF 64 kDa subunit tau • CSTF 64 kDa subunit tau variant • CstF-64T • tauCstF-64
Product Information
Applications
IHC, IP, WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CSTF2T/TauCSTF64 immobilized on solid support.
The epitope recognized by A301-487A maps to a region between residue 566 and 616 of human cleavage stimulation factor 64 kDa subunit, tau variant using the numbering given in entry NP_056050.1 (GeneID 23283).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western blots of immunoprecipitates are performed using an anti-Rabbit IgG Light Chain HRP-conjugated antibody with 5% Normal Pig Serum (Cat. No. S100-020) added to the blocking buffer.
Immunohistochemistry (IHC)1:500 - 1:2000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Immunoprecipitation (IP)6 µg/mg lysate
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-487A · Lot 3 (current lot)Open
Product DatasheetPDF · A301-487A · Lot 3 (current lot)Open
Safety Data SheetPDF · A301-487AOpen
Citations / Publications
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