Bethyl Laboratories
Rabbit anti-CSTF50 IHC Antibody Affinity Purified
Primary
Catalog #IHC-00248
TargetCSTF50
HostRabbit
ClonalityPolyclonal
ReactivityHuman
ApplicationIHC
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Product Description
CSTF50 is one of three subunits that comprise the cleavage stimulation factor (CSTF) complex. CSTF is part of the polyadenylation machinery that adds adenylate residues to the 3’-ends of eukaryotic pre-mRNAs. In the CSTF complex, CSTF50 interacts with CSTF77 (CSTF3), and CSTF64 (CSTF2).
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 75 and 125
Isotype
IgG
Reactivity
Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
250 µg/ml
Target Identity
TargetCSTF50
Antigen Species
Human
Gene ID1477
Gene Symbol
CSTF1
Gene Name
cleavage stimulation factor subunit 1
Uniprot IDQ05048
Protein Name
Cleavage stimulation factor subunit 1
Product Information
Applications
IHC
Buffer
Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to CSTF50 immobilized on solid support.
The epitope recognized by IHC-00248 maps to a region between residue 75 and 125 of human cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa using the numbering given in entry NP_001315.1 (GeneID 1477 ).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
In some cases, the antibody may be diluted further than indicated.
Immunohistochemistry (IHC)1:100 - 1:500
Documents
Safety Data SheetPDF · IHC-00248Open
Product DatasheetPDF · IHC-00248 · Lot 1 (current lot)Open
Product DatasheetPDF · IHC-00248 · Lot 1 (current lot)Open
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