Bethyl Laboratories
Rabbit anti-Cul3 Antibody Affinity Purified
Primary
Catalog #A301-110A
TargetCul3
HostRabbit
ClonalityPolyclonal
ReactivityMouse, Human
ApplicationWB
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Product Description
Cul3 is a member of the cullin group of proteins and functions as a scaffold to bring together ubiquitin E3 ligases, E2 conjugating enzyme, and substrate proteins to mediate the ubiquitination of substrate proteins. Cul3 and its associated ubiquitin ligase activity are essential for mitotic division and required for correct alignment of chromosomes and the completion of cytokinesis. Aurora B is a substrate of Cul3-based E3 ubiquitin ligase activity.
Specifications
Clonality
Polyclonal
Host
Rabbit
Immunogen
Between 718 and C-terminus
Isotype
IgG
Reactivity
Mouse, Human
Format
Whole IgG
Conjugate Type
Unconjugated
Purity
Antigen Affinity Purified
Concentration
1000 µg/ml
Target Identity
TargetCul3
Antigen Species
Human
Gene ID8452
Gene Symbol
CUL3
Gene Name
cullin 3
Uniprot IDQ13618
Protein Name
Cullin-3
Product Information
Applications
WB
Buffer
Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Storage Conditions
2 - 8 °C
Physical State
Liquid
Shelf Life
1 year from date of receipt
Country of Origin
USA
Production & Epitope
Antibody was affinity purified using an epitope specific to Cul3 immobilized on solid support.
The epitope recognized by A301-110A maps to a region between residue 718 and 768 of human cullin 3 using the numbering given in entry NP_003581.1 (GeneID 8452).
Immunoglobulin concentration was determined using Beer’s Law where 1mg/mL IgG has an A280 of 1.4.
Applications
All western blot analysis is performed using 5% Milk-TBST for blocking and as antibody diluent. Primary antibody is incubated overnight.
Western blots of cell lysates are performed using Goat anti-Rabbit IgG Heavy and Light Chain Antibody (A120-101P).
Western Blot (WB)1:2000 - 1:10,000
Documents
Product DatasheetPDF · A301-110A · Lot 2 (current lot)Open
Product DatasheetPDF · A301-110A · Lot 2 (current lot)Open
Safety Data SheetPDF · A301-110AOpen
Citations / Publications
Services
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